Valentrifect™ Stem

iPSC transfection with low stress.

A drop-in replacement for today’s lipid reagents in induced pluripotent stem cells: the same workflow, with much more expression, and cells that stay pluripotent.

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3–100×more GFP-positive cells across six human iPSC linesvs. Lipofectamine™ Stem

6 of 6iPSC lines with higher expression than Lipofectamine™ Stem, at 1× and 2× reagentn = 4 biological replicates

3 sizes100 µL, 300 µL and 1 mL; about 400 transfections per 300 µL in 24-well platesmRNA, plasmid DNA or self-amplifying RNA

Performance data

Higher expression in every iPSC line we tested.

Six human iPSC lines were transfected with GFP mRNA using Valentrifect™ Stem or Lipofectamine™ Stem, each at two reagent amounts.

Bar charts of the ratio of GFP-positive cell fraction, Valentrifect™ Stem over Lipofectamine Stem, at low and high reagent concentration across six iPSC lines — **Ratio of GFP-positive cell fraction, Valentrifect™ Stem / Lipofectamine™ Stem,** at low reagent concentration (top) and high reagent concentration (bottom). Log scale.

Pluripotency & differentiability

Transfected iPSCs stay pluripotent and can still differentiate.

Transfected iPSCsGFP-positive cells after Valentrifect™ Stem mRNA delivery

Core pluripotency factors retained

OCT4SOX2NANOG

No shift vs. untransfected controls and no spontaneous differentiation, the same result as Lipofectamine™ Stem.

Mesoderm

100%

of GFP-positive cells differentiated to mesoderm.

NCAMBrachyury

Ectoderm

Observed

GFP-positive cells differentiated to ectoderm (abbreviated protocol).

PAX6Nestin

Pluripotency by flow cytometry (OCT4, SOX2, NANOG) in GFP-positive cells; lineage markers by flow cytometry after directed differentiation.

All iPSC measurements on this page were made by Forte Biosciences.

Cell health

Gentle on cells. Strong on expression.

Cell viability above 90% in all conditions tested.
No interferon or stress response at 6 h or 24 h across five marker genes, matching untreated cells and Lipofectamine™ Stem.
OCT4, SOX2 and NANOG retained in GFP-positive cells, with no shift versus untransfected controls and no spontaneous differentiation.
Transfected cells still differentiate on demand: 100% of GFP-positive cells differentiated to mesoderm, and ectoderm differentiation was also observed (abbreviated protocol).

Independent testing by Forte Biosciences, including viability. KOLF2 hiPSCs transfected with GFP mRNA; RT-qPCR for stress markers; flow cytometry for pluripotency (OCT4, SOX2, NANOG) and, after directed differentiation, lineage markers.

At a glance

What you get.

ApplicationiPSC transfection

Format100 µL, 300 µL or 1 mL (about 400 transfections per 300 µL in 24-well plates)

PayloadsmRNA, plasmid DNA, self-amplifying RNA

Particle size80 ± 5 nm

StabilityRoom temperature for 15+ days; 4 °C for 1 year

UseResearch use only

Free evaluation sample

See it on your own iPSCs.

We provide evaluation samples so you can compare Valentrifect™ Stem with your current reagent at your own bench.

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For research use only. Not for diagnostic or therapeutic use. Lipofectamine™, MessengerMAX™ and ExpiFectamine™ are trademarks of Thermo Fisher Scientific, which is not affiliated with Valentris.